biorad flamingo stain  (Bio-Rad)

Journal: Current protocols in protein science

Article Title: Protein Detection in Gels Using Fixation UNIT 10.5

doi: 10.1002/cpps.48

Figure Lengend Snippet: Overview of Common Gel Staining Methods

Article Snippet: Representative Common Coomassie Stains table ft1 table-wrap mode="anchored" t5 Table 10.5.4 caption a7 Representative Vendors Stain Limit of Detection Time MS Compatible BioRad Flamingo Fluorescent Gel Stain 0.25–0.5 ng 5 hr yes Oriole Fluorescent Gel Stain 0.5–1 ng 1.5 hr Thermo Fisher CoomassieTM Fluor Orange 8 ng 1 hr yes Krypton Fluorescent Protein Stain 0.25 ng 2 hr 40 min Thermo Fisher; Sigma; BioRad; Lonza SYPRO ® Ruby 0.25 ng 90 min a /18 hr yes Thermo Fisher; Sigma SYPRO ® Orange 4–8 ng ~1 hr yes Thermo Fisher; Lonza SYPRO ® Red 4–8 ng ~1 hr yes SYPRO ® Tangerine 4 ng ~1 hr yes Open in a separate window a The 90 min rapid protocol requires microwaving the gel and staining solution for 3 × 30 sec, or alternatively incubating the gel at 80°C for 30 min followed by subsequent destaining.

Techniques: Staining, Quantitation Assay, Mass Spectrometry, Silver Staining

Journal: Current protocols in protein science

Article Title: Protein Detection in Gels Using Fixation UNIT 10.5

doi: 10.1002/cpps.48

Figure Lengend Snippet: Representative Common Fluorescent Stains

Article Snippet: Representative Common Coomassie Stains table ft1 table-wrap mode="anchored" t5 Table 10.5.4 caption a7 Representative Vendors Stain Limit of Detection Time MS Compatible BioRad Flamingo Fluorescent Gel Stain 0.25–0.5 ng 5 hr yes Oriole Fluorescent Gel Stain 0.5–1 ng 1.5 hr Thermo Fisher CoomassieTM Fluor Orange 8 ng 1 hr yes Krypton Fluorescent Protein Stain 0.25 ng 2 hr 40 min Thermo Fisher; Sigma; BioRad; Lonza SYPRO ® Ruby 0.25 ng 90 min a /18 hr yes Thermo Fisher; Sigma SYPRO ® Orange 4–8 ng ~1 hr yes Thermo Fisher; Lonza SYPRO ® Red 4–8 ng ~1 hr yes SYPRO ® Tangerine 4 ng ~1 hr yes Open in a separate window a The 90 min rapid protocol requires microwaving the gel and staining solution for 3 × 30 sec, or alternatively incubating the gel at 80°C for 30 min followed by subsequent destaining.

Techniques: Staining

Journal: Current protocols in protein science

Article Title: Protein Detection in Gels Using Fixation

doi: 10.1002/0471140864.ps1005s29

Figure Lengend Snippet: Overview of Common Gel Staining Methods

Article Snippet: Representative Common Coomassie Stains table ft1 table-wrap mode="anchored" t5 Table 10.5.4 caption a7 Representative Vendors Stain Limit of Detection Time MS Compatible BioRad Flamingo Fluorescent Gel Stain Oriole Fluorescent Gel Stain 0.25–0.5 ng 0.5–1 ng 5 hr 1.5 hr yes Thermo Fisher Coomassie TM Fluor Orange Krypton Fluorescent Protein Stain 8 ng 0.25 ng 1 hr 2 hr 40 min yes Thermo Fisher; Sigma; BioRad; Lonza SYPRO ® Ruby 0.25 ng 90 min a /18 hr yes Thermo Fisher; Sigma SYPRO ® Orange 4–8 ng ~1 hr yes Thermo Fisher; Lonza SYPRO ® Red SYPRO ® Tangerine 4–8 ng 4 ng ~1 hr ~1 hr yes yes Open in a separate window a The 90 min rapid protocol requires microwaving the gel and staining solution for 3 × 30 sec, or alternatively incubating the gel at 80°C for 30 min followed by subsequent destaining.

Techniques: Staining, Quantitation Assay, Mass Spectrometry, Silver Staining

Journal: Current protocols in protein science

Article Title: Protein Detection in Gels Using Fixation

doi: 10.1002/0471140864.ps1005s29

Figure Lengend Snippet: Representative Common Fluorescent Stains

Article Snippet: Representative Common Coomassie Stains table ft1 table-wrap mode="anchored" t5 Table 10.5.4 caption a7 Representative Vendors Stain Limit of Detection Time MS Compatible BioRad Flamingo Fluorescent Gel Stain Oriole Fluorescent Gel Stain 0.25–0.5 ng 0.5–1 ng 5 hr 1.5 hr yes Thermo Fisher Coomassie TM Fluor Orange Krypton Fluorescent Protein Stain 8 ng 0.25 ng 1 hr 2 hr 40 min yes Thermo Fisher; Sigma; BioRad; Lonza SYPRO ® Ruby 0.25 ng 90 min a /18 hr yes Thermo Fisher; Sigma SYPRO ® Orange 4–8 ng ~1 hr yes Thermo Fisher; Lonza SYPRO ® Red SYPRO ® Tangerine 4–8 ng 4 ng ~1 hr ~1 hr yes yes Open in a separate window a The 90 min rapid protocol requires microwaving the gel and staining solution for 3 × 30 sec, or alternatively incubating the gel at 80°C for 30 min followed by subsequent destaining.

Techniques: Staining